Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

26 July 2020: Lab/In Vitro Research

Vitamin D Attenuates Hypoxia-Induced Injury in Rat Primary Neuron Cells through Downregulation of the Dual Oxidase 1 (DUOX1) Gene

Panpan Cui 12BD , Yan Wang 1AC , Yanzhong Li 1AE* , Lei Ge 3F

DOI: 10.12659/MSM.925350

Med Sci Monit 2020; 26:e925350

Figure 4 Vitamin D attenuated DUOX1-induced injury in rat primary neuron cells. (A, B) The overexpression efficiency of DUOX1 was detected by Q-PCR (A) and western blot (B). (C, D) MDA (C) and SOD (D) levels were detected by biochemical detection. (E, F) ROS level (E) and apoptosis (F) were detected by flow cytometry. (G) Protein expression of NF-κB and cleaved caspase-3 was determined by western blot. *** P<0.001 versus vector; ## P<0.01, ### P<0.001 versus oeDUOX1+vehicle. Vector: Negative control of DUOX1 overexpression; oeDUOX1: DUOX1 overexpression. DUOX1 – dual oxidase 1; Q-PCR – quantitative polymerase chain reaction; MDA – malondialdehyde; SOD – superoxide dismutase; ROS – reactive oxygen species.

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750