Figure 3 The expression of BDNF in the brains of mice sacrificed 24 hours after exposure to 2D-SWE. The relative mRNA expression levels of BDNF was detected by qRT-PCR (A). The protein expression levels of BDNF were quantified by western blots (B). The densitometric analysis of each band was performed using Image-Pro Plus 6.0, and the protein expression levels were normalized to those of actin. Data are presented as the mean±SD from 3 independent experiments. Differences in measurement data were compared with one-way ANOVA method. * P<0.05; ** P<0.01, and NS – no significance (P>0.05). BDNF – brain-derived neurotrophic factor; 2D-SWE – 2-dimensional shear wave elastography; mRNA – messenger RNA; qRT-PCR – quantitative real-time polymerase chain reaction; SD – standard deviation.