28 June 2020: Lab/In Vitro Research
Meteorin-Like (METRNL) Attenuates Myocardial Ischemia/Reperfusion Injury-Induced Cardiomyocytes Apoptosis by Alleviating Endoplasmic Reticulum Stress via Activation of AMPK-PAK2 Signaling in H9C2 Cells
Ling Xu A , Yinlian Cai CD , Yaoguo Wang B , Chaoxiang Xu C**DOI: 10.12659/MSM.924564
Med Sci Monit 2020; 26:e924564
Figure 5 PAK2 silencing blocked the inhibitive effect of METRNL overexpression on cell apoptosis induced by OGD/R. (A) The apoptosis rate of H9C2 cells was analyzed by TUNEL assay (200x magnification). (B) The expressions of Bcl2, Bax, cleaved caspase-3, pre caspase-3, caspase-12, and PARP were evaluated by western blot. Error bars represent the mean±SEM from 3 independent experiments. *** P<0.001 versus Control; ## P<0.01, ### P<0.001 versus OGD/R 12 hours; @ P<0.05, @@ P<0.01 versus OGD/R+Ov-METRNL. PAK2 – p21-activated kinase 2; METRNL – Meteorin-like; OGD/R – oxygen-glucose deprivation and reperfusion; TUNEL – terminal deoxynucleotidyl transferase dUTP nick end labeling immunohistochemical; PARP – poly ADP-ribose polymerase; SEM – standard error of the mean; Ov-METRNL – overexpression-METRNL.