Figure 6 Effect of SIRT1 on NF-κB signaling pathway in OGD-induced neuronal cells. Primary rat neuronal cells were transfected with SIRT1-plasmid/SIRT1-siRNA or control-plasmid/control-siRNA for 48 h, then the cells were treated with OGD for 48 h. (A) p-p65 and p65 protein levels in neurons transfected with SIRT1-plasmid or control-plasmid for 48 h were determined by Western blot assay. (B) The ratio of p-p65/p65 in neurons transfected with SIRT1-plasmid or control-plasmid for 48 h was calculated and presented. (C) p-p65 and p65 protein levels in neurons transfected with SIRT1-siRNA or control-siRNA for 48 h were determined by Western blot assay. (D) The ratio of p-p65/p65 in neurons transfected with SIRT1-siRNA or control-siRNA for 48 h was calculated and presented. Control: cells without any treatment; OGD: cells were subjected to OGD treatment; OGD+control-plasmid: cells were transfected with control-plasmid and then subjected to OGD treatment; OGD+SIRT1-plasmid: cells were transfected with SIRT1-plasmid and then subjected to OGD treatment; OGD+control-siRNA: cells were transfected with control-siRNA and then subjected to OGD treatment; OGD+SIRT1-siRNA: cells were transfected with SIRT1-siRNA and then subjected to OGD treatment. The results are presented as the mean±SD. ** P<0.01 vs. control group; # P<0.05, ## P<0.01 vs. OGD group. OGD – oxygen and glucose deprivation; SIRT1 – sirtuin-1.