Figure 2 SIRT1 expression was downregulated in the OGD-induced primary rat neurons. (A) The protein expression levels of the related molecular markers S100B and NSE during the development of HIE were determined by Western blot assay. (B) SIRT1 protein levels were determined by Western blot assay. (C) SIRT1 mRNA gene expression was determined by qRT-PCR. Control: cells without any treatment; OGD: cells were stimulated with OGD. The results showed as the mean±SD. ** P<0.01 vs. control group. SIRT1 – sirtuin-1; OGD – oxygen and glucose deprivation.