Figure 3 The results of differential expression genes analysis and functional enrichment analysis between low mRNAsi PRAD and high mRNAsi PRAD. A total of 2462 genes including 2029 downregulated ones and 433 upregulated ones were identified as DEGs between low mRNAsi PRAD and high mRNAsi PRAD, illustrating in heatmap (A) and volcano plot (B). GO (C) and KEGG (D) terms including muscle system process, collagen–containing extracellular matrix, passive transmembrane transporter activity and neuroactive ligand–receptor interaction were identified as significantly enriched items in stemness-related DEGs, illustrating in bubble plot. PRAD – prostate adenocarcinoma; mRNAsi – mRNA expression-based stemness index; DEGs – differential expressed genes; GO – Gene Ontology; KEGG – Kyoto Encyclopedia of Genes and Genomes.