22 July 2020: Clinical Research
Phospholipase Cɛ Regulates Prostate Cancer Lipid Metabolism and Proliferation by Targeting AMP-Activated Protein Kinase (AMPK)/Sterol Regulatory Element-Binding Protein 1 (SREBP-1) Signaling Pathway
Yongbo Zheng 1ABCDEF* , Jiajia Jin 2ABCDF* , Yingying Gao 3ACF , Chunli Luo 4AG , Xiaohou Wu 1AG* , Jiayu Liu 1ABCDE*DOI: 10.12659/MSM.924328
Med Sci Monit 2020; 26:e924328
Figure 6 Silencing PLCɛ suppresses proliferation and lipid metabolism through AMPK/SREBP-1. (A–D) The protein levels of total-AMPKα, p-AMPKα, and SREBP-1 of PCa cells with different treatments, as determined by Western blot. (E, F) Nile red staining assay was used to assess lipid droplets content of PCa cells with different treatments. (G–J) Fluorescence of Ki-67 and colony formation assay were used to examine proliferation of PCa cells with different treatments. *** p<0.001, ** p<0.01, * p<0.05, ns – no statistical significance; NC – negative control. (400×, 200 μm bar).