28 July 2020>: Lab/In Vitro Research
CircKIAA0907 Retards Cell Growth, Cell Cycle, and Autophagy of Gastric Cancer and Inhibits Tumorigenesis via the miR-452-5p/KAT6B Axis
Lingyu Zhu 1BCDE , Chunfei Wang 1CDEF , Shengquan Lin 2BCDE , Lei Zong 1ABCD*DOI: 10.12659/MSM.924160
Med Sci Monit 2020; 26:e924160
Figure 6 CircKIAA0907 worked as a tumor repressor in gastric cancer (GC) progression via the miR-452-5p-mediated KAT6B upregulation. (A, B) KAT6B was measured using western blot in HGC27 and AGS cells transfected with anti-miR-NC, anti-miR-452-5p, anti-miR-452-5p+si-NC, or anti-miR-452-5p+si-KAT6B. (C, D) Examination of proliferation ability in transfected cells implemented through 3-(4, 5-dimethylthiazol-2-y1)-2, 5-diphenyl tetrazolium bromide (MTT) assay. (E–H) Flow cytometry and western blot to evaluate cell apoptosis. (I, J) Cell cycle analysis using flow cytometry. (K, L) Cellular autophagy assessed utilizing western blot. * P<0.05.