Figure 5 CircKIAA0907 sponged miR-452-5p to elevate expression of its target KAT6B. (A) The starbase v2.0 was utilized for analyzing the binding sites of KAT6B 3′-UTR and miR-452-5p. (B, C) The dual-luciferase reporter assay was used to determine whether miR-452-5p interacted with KAT6B. (D) The impact of anti-miR-452-5p on KAT6B was assayed using western blot. (E, F) Analysis of KAT6B level in gastric cancer (GC) tissues (E) and cells (F) via western blot. (G) After the respective transfection of circKIAA0907, circKIAA0907+miR-452-5p, or relative controls in HGC27 and AGS cells, the protein expression of KAT6B was determined via western blot. * P<0.05.