Figure 4 Increase of miR-452-5p reversed the inhibition of circKIAA0907 on the progression of gastric cancer (GC) cells. (A, B) After transfection of vector, circKIAA0907, circKIAA0907+miR-NC, and circKIAA0907+miR-452-5p in HGC27 and AGS cells, the expression of miR-452-5p was assayed via quantitative real-time polymerase chain reaction (qRT-PCR). (C, D) 3-(4, 5-Dimethylthiazol-2-y1)-2, 5-diphenyl tetrazolium bromide (MTT) assay was used for measuring proliferation ability in transfected cells. (E–H) Cell apoptosis was assessed with flow cytometry and western blot. (I, J) The percentage of each phase in transfected GC cells was examined by cell cycle detection. (K, L) Evaluation of autophagy was carried out by detecting associated proteins after western blot. * P<0.05.