Figure 3 CircKIAA0907 could sponge miR-452-5p in gastric cancer (GC) cells. (A, B) Expression level of circKIAA0907 was analyzed by quantitative real-time polymerase chain reaction (qRT-PCR) after biotinylated-circKIAA0907 pull-down assay in HGC27 and AGS cells transfected with vector or circKIAA0907. (C, D) qRT-PCR was used to quantify the relative expression of candidate micro(mi)RNAs in HGC27 and AGS cells after administration of biotinylated-circKIAA0907 pull-down. (E) Binding sites of circKIAA0907 and miR-452-5p presented after analysis of circBank. (F, G) Pull-down assay was executed to prove the capture of circKIAA0907 by miR-452-5p. (H, I) Luciferase activities of transfected HGC27 and AGS cells were determined using the dual-luciferase reporter system. (J, K) MiR-452-5p level was examined using qRT-PCR after actinomycin D treatment at 0, 12, and 24 h in GC cells transfected with vector or circKIAA0907. (L, M) miR-452-5p was measured in GC tissues (L) and cells (M) by qRT-PCR. (N, O) MiR-452-5p level was determined through qRT-PCR after GC cells were transfected with vector or circKIAA0907. * P<0.05.