Figure 2 Overexpression of circKIAA0907 triggered proliferation and autophagy inhibition, cell cycle arrest, and apoptosis promotion of gastric cancer (GC) cells in vitro. (A, B) Evaluation of circKIAA0907 overexpression efficiency was conducted via quantitative real-time polymerase chain reaction (qRT-PCR) after transfection of vector or circKIAA0907. (C, D) Proliferation analysis in transfected GC cells was performed by 3-(4, 5-dimethylthiazol-2-y1)-2, 5-diphenyl tetrazolium bromide (MTT) assay. (E, F) Apoptosis rate of transfected cells was determined using flow cytometry. (G, H) Apoptosis-related proteins were assayed using western blot. (I, J) Cell cycle was analyzed via flow cytometry. (K, L) Western blot was used for detecting the autophagy-associated markers. * P<0.05.