09 August 2020>: Clinical Research
Long Non-Coding RNA SNHG8 Plays a Key Role in Myocardial Infarction Through Affecting Hypoxia-Induced Cardiomyocyte Injury
Yue Zhang 1ABCDEFG , Yunfei Bian 2ABCDEG*DOI: 10.12659/MSM.924016
Med Sci Monit 2020; 26:e924016
Figure 8 Effects of lncRNA SNHG8 on NF-κB signal pathway in hypoxia-induced cardiomyocytes. (A, C) Western blotting analysis was used to determine p-p65 and p65 protein expression in H9c2 cells. (B, D) p-p65/p65 ratio in hypoxia-induced cardiomyocytes following transfection. Control: cells without any treatment; Hypoxia: H9c2 cells were cultured under hypoxic conditions (94% N2, 5% CO2, and 1% O2) for 48 h; Hypoxia+control-plasmid: H9c2 cells were transfected with control-plasmid for 48 h under hypoxic conditions; Hypoxia+SNHG8-plasmid: H9c2 cells were transfected with SNHG8-plasmid for 48 h under hypoxic conditions. Hypoxia+control-shRNA: H9c2 cells were transfected with control-shRNA for 48 h under hypoxic conditions; Hypoxia+SNHG8-shRNA: H9c2 cells were transfected with SNHG8-shRNA for 48 h under hypoxic conditions. ## P<0.01 vs. Control group; & P<0.05 vs. Hypoxia+control-plasmid; ** P<0.01 vs. Hypoxia+control-shRNA.