09 August 2020>: Clinical Research
Long Non-Coding RNA SNHG8 Plays a Key Role in Myocardial Infarction Through Affecting Hypoxia-Induced Cardiomyocyte Injury
Yue Zhang 1ABCDEFG , Yunfei Bian 2ABCDEG*DOI: 10.12659/MSM.924016
Med Sci Monit 2020; 26:e924016
Figure 4 Effects of up-regulating lncRNA SNHG8 on the secretion of inflammatory factors in hypoxia-induced cardiomyocytes. (A–C) Secretion of IL-1β, TNF-α, and IL-6 in transfected hypoxia-induced cardiomyocytes were measured by ELISA. (D–F) Relative expression levels of IL-1β, TNF-α, and IL-6 in transfected hypoxia-induced cardiomyocytes were determined by qRT-PCR analysis. Control: cells without any treatment; Hypoxia: H9c2 cells were cultured under hypoxic conditions (94% N2, 5% CO2, and 1% O2) for 48 h; Hypoxia+control-plasmid: H9c2 cells were transfected with control-plasmid for 48 h under hypoxic conditions; Hypoxia+SNHG8-plasmid: H9c2 cells were transfected with SNHG8-plasmid for 48 h under hypoxic conditions. ## P<0.01 vs. Control group; &, && P<0.5, 0.01 vs. Hypoxia+control-plasmid.