Figure 3 Effects of SNHG8-plasmid on the cell viability and apoptosis in hypoxia-induced cardiomyocytes. (A) Cell viability in hypoxia-induced cardiomyocytes following transfection was measured by MTT assay. (B, C) Flow cytometry analysis was used to determine the apoptotic rate of hypoxia-induced cardiomyocytes. (D, E) Western blotting analysis was performed to detect relative expression of cleaved-Caspase3 protein. Control: cells without any treatment; Hypoxia: H9c2 cells were cultured under hypoxic conditions (94% N2, 5% CO2, and 1% O2) for 48 h; Hypoxia+control-plasmid: H9c2 cells were transfected with control-plasmid for 48 h under hypoxic conditions; Hypoxia+SNHG8-plasmid: H9c2 cells were transfected with SNHG8-plasmid for 48 h under hypoxic conditions. ## P<0.01 vs. Control group; &, && P<0.5, 0.01 vs. hypoxia+control-plasmid.