Figure 2 Effects of SNHG8-plasmid on cell injury in hypoxia-induced cardiomyocytes. (A) Transfection efficiency was evaluated by qRT-PCR assay. (B) Release of CK-MB was measured by using a CK-MB Detection Kit. (C) Release of cTnI was measured by using a cTnI Detection Kit. (D) A mitochondrial activity assay kit was used to detect mitochondrial viability of hypoxia-induced cardiomyocytes. Control: cells without any treatment; plasmid-control: H9c2 cells were transfected with control-plasmid for 48 h; SNHG8-plasmid: H9c2 cells were transfected with SNHG8-plasmid for 48 h; Hypoxia: H9c2 cells were cultured under hypoxic conditions (94% N2, 5% CO2, and 1% O2) for 48 h; Hypoxia+control-plasmid: H9c2 cells were transfected with control-plasmid for 48 h under hypoxic conditions; Hypoxia+SNHG8-plasmid: H9c2 cells were transfected with SNHG8-plasmid for 48 h under hypoxic conditions. ** P<0.01 vs. control-plasmid; ## P<0.01 vs. Control group; &, && P<0.5, 0.01 vs. Hypoxia+control-plasmid.