Figure 5 miR-29a targeted the BCL2L11 gene in HEK-293 cells. (A) The sequences of wild-type and mutant-type vectors and putative binding sites of miR-29a within BCL2L11 mRNA. (B) The relative luciferase activities were detected in HEK-293 cells co-transfected with vectors containing the wild- or mutant-type of BCL2L11 together with miR-29a mimics or miR-nc. (C, D) The relative mRNA and protein expression of BCL2L11 after transfection of miR-29a mimic or si-miR-29a. (E) The expression level of BCL2L11 mRNA was measured by RT-qPCR in the sample pulled down by biotinylated miR-29a. (F) The correlation between miR-29a and BCL2L11 was analyzed by Pearson analysis. * P<0.05 vs. miR-nc or biotin-nc, ** P<0.01 vs. miR-nc, # P<0.01 vs. si-nc.