Figure 3 Reciprocal inhibition between lncRNA NEAT1 and miR-29a in HEK-293 cells. (A) The sequences of wild-type and mutant-type vectors and putative binding sites of miR-29a within lncRNA NEAT1 mRNA. (B) The relative luciferase activities were detected by dual-luciferase reporter assay in HEK-293 cells co-transfected with wild-type and mutant-type vectors together with miR-29a mimics and miR-nc. (C) The expression level of lncRNA NEAT1 mRNA was measured by RT-qPCR in the sample pulled down by biotinylated miR-29a. (D) The relative expression of miR-29a in PC-12 cells after infection with Ad-si-NEAT1 or si-nc. * P<0.05 vs. miR-nc, ** P<0.01 vs. biotin-nc or si-nc.