09 July 2020: Lab/In Vitro Research
Long Non-Coding RNA (lncRNA) Small Nucleolar RNA Host Gene 15 (SNHG15) Alleviates Osteoarthritis Progression by Regulation of Extracellular Matrix Homeostasis
Yunping Chen 1AB* , Hongna Guo 1AB* , Lang Li 23A , Dingsu Bao 24B , Feng Gao 3C , Qiang Li 3D , Qi Huang 3D , Xin Duan 2F , Zhou Xiang 2AG*DOI: 10.12659/MSM.923868
Med Sci Monit 2020; 26:e923868
Figure 6 SNHG15 indirectly regulates KLF4 expression by miR-7. (A) The distribution of SNHG15 in subcellular fractions of chondrocytes was evaluated by qRT-PCR. U6 and GAPDH served as nuclear and cytoplasmic markers, respectively. (B) Correlation analysis results showed that SNHG15 was negatively correlated with miR-7. (C, D) Luciferase reporter assay and RIP confirmed that SNHG15 was a target of miR-7. (E, F) SNHG15 overexpression significantly promoted the expression levels of KLF4 in IL-1β-induced chondrocytes as shown by Western blot. (G, H) MiR-7 significantly reversed the elevated expression of SNHG15 on KLF4 expression in IL-1β-induced chondrocytes as shown by Western blot. **** p<0.0001.