09 July 2020: Lab/In Vitro Research
Long Non-Coding RNA (lncRNA) Small Nucleolar RNA Host Gene 15 (SNHG15) Alleviates Osteoarthritis Progression by Regulation of Extracellular Matrix Homeostasis
Yunping Chen 1AB* , Hongna Guo 1AB* , Lang Li 23A , Dingsu Bao 24B , Feng Gao 3C , Qiang Li 3D , Qi Huang 3D , Xin Duan 2F , Zhou Xiang 2AG*DOI: 10.12659/MSM.923868
Med Sci Monit 2020; 26:e923868
Figure 5 SNHG15 overexpression inhibited ECM degradation and promoted chondrocyte formation in an experimental OA model. (A) The morphology changes of cartilage tissues in the experimental OA model injected by SMHG15 overexpression using Safranin-O and fast green staining. (B, C) Cartilage destruction was assessed according to the OARSI and Mankin scores. (D) Representative images of immunohistochemistry of COL2A1 and Aggrecan in cartilage tissues of the experimental OA model injected by SMHG15 overexpression. Scale bar: 50 μm. Magnification: 200×. (E) Quantitative results of immunohistochemistry for COL2A1 and Aggrecan. (F) COL2A1 and Aggrecan expression in cartilage tissues of experimental OA model injected by SMHG15 overexpression using qRT-PCR. (G, H) MMP3 and ADAMTS5 expression in cartilage tissues of experimental OA model injected by SMHG15 overexpression using western blot. * p<0.05; ** p<0.01, *** p<0.001; **** p<0.0001.