09 July 2020: Lab/In Vitro Research
Long Non-Coding RNA (lncRNA) Small Nucleolar RNA Host Gene 15 (SNHG15) Alleviates Osteoarthritis Progression by Regulation of Extracellular Matrix Homeostasis
Yunping Chen 1AB* , Hongna Guo 1AB* , Lang Li 23A , Dingsu Bao 24B , Feng Gao 3C , Qiang Li 3D , Qi Huang 3D , Xin Duan 2F , Zhou Xiang 2AG*DOI: 10.12659/MSM.923868
Med Sci Monit 2020; 26:e923868
Figure 4 SHNG15 was downregulated in OA knee cartilage tissues and its overexpression inhibited ECM degradation and promoted chondrocyte formation in IL-1β-induced chondrocytes. (A) SNHG15 expression in human OA and normal cartilage tissues using GSE114007. (B) qRT-PCR showing the expression of SNHG15 in human OA and normal cartilage tissues. (C) Transfection efficiency of SNHG15 overexpression vector in chondrocytes. (D) Immunofluorescence assay results showing the expression levels of COL2A1 and Aggrecan in IL-1β-stimulated chondrocytes transfected by SNHG15 overexpression. (E, F) Western blot assay showing MMP3 and ADAMTS5 expression in IL-1β-stimulated chondrocytes transfected by SNHG15 overexpression. (G) The Ki67 expression in IL-1β-stimulated chondrocytes transfected by SNHG15 overexpression using immunofluorescence assay. *** p<0.001, **** p<0.0001.