09 July 2020>: Lab/In Vitro Research
Long Non-Coding RNA (lncRNA) Small Nucleolar RNA Host Gene 15 (SNHG15) Alleviates Osteoarthritis Progression by Regulation of Extracellular Matrix Homeostasis
Yunping Chen 1AB* , Hongna Guo 1AB* , Lang Li 23A , Dingsu Bao 24B , Feng Gao 3C , Qiang Li 3D , Qi Huang 3D , Xin Duan 2F , Zhou Xiang 2AG*DOI: 10.12659/MSM.923868
Med Sci Monit 2020; 26:e923868
Figure 2 Low KLF4 expression in human OA knee cartilage tissues and IL-1β-stimulated OA chondrocytes. (A) Comparison of expression levels of KLF4 between human OA and normal cartilage tissues in GSE114007 dataset. (B) qRT-PCR showing KLF4 expression in IL-1β-stimulated chondrocytes. (C) Protein-protein interaction network of KLF4. (D, E) Functional enrichment analysis results of co-expressed proteins of KLF4. (F) qRT-PCR showing the transfection efficiency of KLF4 overexpression or knockdown in chondrocytes. (G) The expression levels of MMP3, ADAMTS5, COL2A1, and Aggrecan in IL-1β-stimulated chondrocytes transfected by KLF4 overexpression as shown by qRT-PCR. * p<0.05; ** p<0.01, *** p<0.001; **** p<0.0001.