Figure 5 AKR1B1 interacted with NORAD. (A) The interplay between NORAD and AKR1B1 was detected by RIP assay. The expression of AKR1B1 in glioma cell lines (GSC11, M059J, U251, and T98G) was measured by (B) western blotting and (C) RT-qPCR, respectively. Three independent experiments were carried out (n=3). ** P<0.01, *** P<0.001 versus A735. The level of AKR1B1 in U251 cells was examined using (D) western blotting and (E) RT-qPCR after transfection with si-NORAD-2. All experiments were repeated 3 times independently (n=3). ** P<0.01 versus si-NC. NORAD – noncoding RNA activated by DNA damage; RIP – RNA-binding protein immunoprecipitation; RT-qPCR – reverse transcription-quantitative polymerase chain reaction; si-NORAD – small interfering RNAs (siRNAs) against NORAD; si-NC – negative control for NORAD siRNA.