21 July 2020>: Lab/In Vitro Research
Silencing of Long Non-Coding RNA (LncRNA) Non-Coding RNA Activated by DNA Damage (NORAD) Inhibits Proliferation, Invasion, Migration, and Promotes Apoptosis of Glioma Cells via Downregulating the Expression of AKR1B1
Lun Luo ABE* , Chuan Chen ACD , Haiyong He DFG , Meiqin Cai BCFG , Cong Ling BCEDOI: 10.12659/MSM.922659
Med Sci Monit 2020; 26:e922659
Figure 2 NORAD silencing inhibited proliferation, invasion, and migration of glioma cells. Cell proliferation was examined using (A) CCK-8 and (B) colony formation assay. (C) The invasion ability of U251 cells was evaluated using Transwell assay. (magnification 200×). (D) Quantification of cell invasion. (E) Representative images and (F) relative quantification of cell migration, as measured using wound healing assay. (magnification 100×). All experiments were repeated 3 times independently (n=3). *** P<0.001 vs. si-NC. NORAD – noncoding RNA activated by DNA damage; CCK-8 – Cell Counting Kit-8; si-NC – negative control for NORAD siRNA.