16 August 2020: Animal Study
Extract EGb761 Attenuates Bleomycin-Induced Experimental Pulmonary Fibrosis in Mice by Regulating the Balance of M1/M2 Macrophages and Nuclear Factor Kappa B (NF-κB)-Mediated Cellular Apoptosis
Ling Pan AD* , Yuehong Lu BCE , Zhanhua Li BCEF , Yuping Tan BF , Hongmei Yang BF , Ping Ruan CDF , Ruixiang Li BFDOI: 10.12659/MSM.922634
Med Sci Monit 2020; 26:e922634
Figure 5 EGb761 reduced pulmonary fibrosis (PF) by regulating apoptosis factors in mouse PF model after treating with bleomycin (BLM) or EGb761 for 28 days. (A) Expressions of phosphorylated nuclear factor (NF)-κB (p65) were validated by western blot. The messenger ribonucleic acid and protein levels of apoptosis factors including B-cell lymphoma X protein (Bax), B-cell lymphoma (Bcl)-2, caspase-3, and caspase-9 in lung tissues of mice from each group were validated by real-time polymerase chain reaction (B) and western blotting (C). Glyceraldehyde 3-phosphate (GAPDH) and tubulin served as the reference control and loading control, respectively. * P<0.05 vs. normal group; # P<0.05 vs. BLM group.