Figure 4 Rab7 promotes the proliferation, invasion and migration of GC cells through PI3K/AKT signaling pathway. (A) Detection of PI3K and AKT protein expression in GC cell lines by Western blot analysis. β-actin was used as loading control. (B, C) The enhancement of Rab7-overexpressing on cell proliferation, invasion and migration was weakened by LY294002. Data represent the mean±SD of 3 independent experiments. * P<0.05; NS – no statistical significance, n=5 random fields, by 2-tailed t test).