Figure 1 Upregulation of Rab7 in carcinoma tissues was associated with the prognosis. (A) Expression of Rab7 in carcinoma tissues and adjacent tissues was detected by IHC. Scale bar, 50 μm. The chart shows that the score of Rab7 in GC is significantly higher than that of NT (* P<0.05, by 2-tailed t test, n=115). (B, C) The results of Western blot and qRT-PCR showed the expression level of Rab7 protein and mRNA in cancer tissues and adjacent tissues. β-actin was used as load control (n=8). (D) According to the staining score, the high-expression group and low-expression group of Rab7 were identified. Kaplan-Meier analysis showed that the overall survival in patients(n=69) with high Rab7 expression was significantly shorter than that(n=40) with low Rab7 expression (P=0.015, by log-rank test). (E) The Rab7 expression of normal tissues and 6 GC cell lines were analyzed by Western blot analysis. β-actin was used as a loading control.