05 July 2020>: Animal Study
Therapeutic Effect of Berberine on Insomnia Rats by ErbB Signaling Pathway
Qingquan Wang 1ABE , Xiaojuan Ren 1C , Xingping Zhang 1DG* , Guanying Wang 1D , Hongxia Xu 1C , Ning Deng 2F , Tao Liu 1D , Zhipeng Peng 1BDOI: 10.12659/MSM.921831
Med Sci Monit 2020; 26:e921831
Figure 3 Berberine regulates gene expression in hippocampus of insomnia rats. (A) Transcription group sequencing identified differentially expressed genes (volcanic and thermal maps) in insomniac rats compared with blank control rats. Red nodes represent upregulated genes and blue nodes represent downregulated genes. (B) The connectivity of disordered genes was screened by PPI network. The larger the node, the higher the connectivity of the gene. Red nodes represent upregulated genes and green nodes represent downregulated genes. (C) Effects of berberine and diazepam on the expression of disordered genes in insomniac rats. (D) qPCR was used to verify the expression of core genes in rats. (E) Western blot was used to verify the expression of core genes in rats. ** P<0.01, *** P<0.001 versus model group, # P<0.05, ## P<0.01 versus diazepam group. PPI – protein–protein interaction; qPCR – quantitative polymerase chain reaction.