Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

15 June 2020: Clinical Research

LncRNA LINC00152 Increases the Aggressiveness of Human Retinoblastoma and Enhances Carboplatin and Adriamycin Resistance by Regulating MiR-613/Yes-Associated Protein 1 (YAP1) Axis

Ying Wang CDE* , Danli Xin ABCE , Lei Zhou BCDF

DOI: 10.12659/MSM.920886

Med Sci Monit 2020; 26:e920886

Figure 3 LINC00152 regulated proliferation, invasion, apoptosis and autophagy of retinoblastoma cells by targeting miR-613. (A–H) Y79 and Weri-RB-1 cells were transfected with si-NC, si-LINC00152#1, anti-miR-NC, anti-miR-613, or si-LINC00152#1+anti-miR-613, untransfected cells as internal control. (A, B) The expression level of miR-613 in transfected Y79 and Weri-RB-1 cells was assessed by RT-qPCR assay. (C, D) CCK-8 assay was performed to evaluate retinoblastoma cell viability at the indicated time points after transfection. (E, F) Cell apoptosis rate and invasion ability were detected in Y79 and Weri-RB-1 cells by flow cytometry and Transwell assays, respectively. (G, H) The protein expression levels of p62, LC3-I, LC3-II and Beclin-1 were examined in Y79 and Weri-RB-1 cells with western blot assay. * P<0.05; ** P<0.01. RT-qPCR – real-time quantitative polymerase chain reaction; CCK-8 – Cell Counting Kit-8.

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750