Scimago Lab
powered by Scopus
call: +1.631.470.9640
Mon-Fri 10 am - 2 pm EST


eISSN: 1643-3750

Published: 2019-04-20

Integrative Analysis of Long Noncoding RNA (lncRNA), microRNA (miRNA) and mRNA Expression and Construction of a Competing Endogenous RNA (ceRNA) Network in Metastatic Melanoma

Li-Xin Wang, Chuan Wan, Zheng-Bang Dong, Bai-He Wang, Hong-Ye Liu, Yang Li

(Department of Dermatology, Affiliated Hospital of Qingdao University, Qingdao, Shandong, China (mainland))

Med Sci Monit 2019; 25:2896-2907

DOI: 10.12659/MSM.913881

Worldwide, metastatic melanoma of the skin has an aggressive course with high morbidity and mortality. Therefore, an increased understanding of the pathogenesis of metastatic melanoma has gained increasing attention, including the role of epigenetic modification and competing endogenous RNA (ceRNA). This study aimed to used bioinformatics data to undertake an integrative analysis of long noncoding RNA (lncRNA), microRNA (miRNA) and mRNA expression to construct a ceRNA network in metastatic melanoma.
Data from the Cancer Genome Atlas (TCGA), the Gene Ontology (GO) database, and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway were analyzed. There were 471 cases that included 103 primary solid tumors and 368 cases of metastatic melanoma that included transcriptome sequencing data (including lncRNA and mRNA); 452 cases had miRNA sequencing data.
Analysis of chip data identified 85 6 mRNAs, 67 miRNAs, and 250 lncRNAs that were differentially expressed in cases of metastatic melanoma, of which 25 miRNAs, 18 lncRNAs, and 18 mRNAs participated in the formation of ceRNAs. Survival analysis identified seven differentially expressed mRNAs, five differentially expressed miRNAs (miRNA-29c, miRNA-100, miR-142-3p, miR-150, miR-516a-2), and six differentially expressed lncRNAs (AC068594.1, C7orf71, FAM41C, GPC5-AS1, MUC19, LINC00402) that were correlated with survival time in patients with metastatic melanoma.
Bioinformatics data and integrative analysis identified lncRNA, miRNA, and mRNA expression to construct a ceRNA and patient survival network in metastatic melanoma. These findings support the need for further studies on the mechanisms involved in the regulation of metastatic melanoma by ceRNAs.

Keywords: 1-Acylglycerol-3-Phosphate O-Acyltransferase, MicroRNAs, NM23 Nucleoside Diphosphate Kinases, RNA Caps, RNA, Long Noncoding

Related Articles (0)

Coming Soon...

Published: 2019-04-19

Effects of Sleep Deprivation (SD) on Rats via ERK1/2 Signaling Pathway

Li Wang, Youyi Gu, Jingjing Zhang, Li Gong

Med Sci Monit 2019; 25:2886-2895

DOI: 10.12659/MSM.913839

Published: 2019-04-19

Cost-Effectiveness Analysis of Integrated Care in Management of Advanced Chronic Obstructive Pulmonary Di...

Ewa Bandurska, Iwona Damps-Konstańska, Piotr Popowski, Tadeusz Jędrzejczyk, Piotr Janowiak, Katarzyna Świętnicka, Marzena Zarzeczna-Baran, Ewa Jassem

Med Sci Monit 2019; 25:2879-2885

DOI: 10.12659/MSM.913358