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Plant-Animal Research Competition

Four Awards ($1,500 each) + publication expenses

We are announcing a competition for external investigator reports dealing with plant-animal communications, which influence animal/human tissues. Eligible studies can vary in their methodology, but state-of-the-art research submissions are encouraged.

Awards Guidelines and Program:
  1. Four Awards granted ($1,500 each) + reimbursement of publication expenses
  2. All reports prepared according to... read more


Published: 2014-12-17

Proteomic Profile in Glomeruli of Type-2 Diabetic KKAy Mice using 2-Dimensional Differential Gel Electrophoresis

Xiaodan Liu, Gang Yang, Qiuling Fan, Lining Wang

(Department of Nephrology, First Affiliated Hospital, China Medical University, Shenyang, Liaoning, China (mainland))

Med Sci Monit 2014; 20:2705-2713

DOI: 10.12659/MSM.893078


Background: Diabetic nephropathy (DN) is a leading cause of end-stage renal disease. To search for glomerular proteins associated with early-stage DN, glomeruli of spontaneous type 2 diabetic KKAy mice were analyzed by 2-dimensional differential gel electrophoresis (2D-DIGE).
Material and Methods: Glomeruli of 20-week spontaneous type 2 diabetic KKAy mice and age-matched C57BL/6 mice were isolated by kidney perfusion with magnetic beads. Proteomic profiles of glomeruli were investigated by using 2D-DIGE and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Western blot analysis was used to confirm the results of proteomics. Immunohistochemical and semi-quantitative analysis were used to confirm the differential expression of prohibitin and annexin A2 in glomeruli.
Results: We identified 19 differentially expressed proteins – 17 proteins were significantly up-regulated and 2 proteins were significantly down-regulated in glomeruli of diabetic KKAy mice. Among them, prohibitin and annexin A2 were up-regulated and Western blot analysis validated the same result in proteomics. Immunohistochemical analysis also revealed up-regulation of prohibitin and annexin A2 in glomeruli of KKAy mice.
Conclusions: Our findings suggest that prohibitin and annexin A2 may be associated with early-stage DN. Further functional research might help to reveal the pathogenesis of DN.

Keywords: Diabetic Nephropathies, Kidney Glomerulus, Proteomics, Two-Dimensional Difference Gel Electrophoresis




Published: 2014-12-17

Postural Sway and Motor Control in Trans-Tibial Amputees as Assessed by Electroencephalography during Eig...


Jerrold Scott Petrofsky, Iman Akef Khowailed

Med Sci Monit 2014; 20:2695-2704

DOI: 10.12659/MSM.891361

Published: 2014-12-16

The Role of Thyroid and Parathyroid Metabolism Disorders in the Etiology of Sudden Onset Dizziness


Ugur Lok, Sinan Hatipoglu, Umut Gulacti, Abdullah Arpaci, Nurettin Aktas, Tayfun Borta

Med Sci Monit 2014; 20:2689-2694

DOI: 10.12659/MSM.891305