IF 2014

Published: 2015-10-05

Effect of Inducible Co-Stimulatory Molecule siRNA in Cerebral Infarction Rat Models

Yingquan Luo, Yu Yang, Hui Zhang, Ting Zhang, Yina Wang, Shengyu Tan, Yan Xu, Dan Li, Ling Ye, Ping Chen

(Department of Geriatrics, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China (mainland))

Med Sci Monit 2015; 21:3003-3007

DOI: 10.12659/MSM.894477

BACKGROUND: T cell-induced inflammatory response and related cytokine secretion at the injury site may participate in the pathogenesis of cerebral infarction. Recent studies established inducible co-stimulatory molecule (ICOS) as a novel T cell-related factor for its activation and functions. We thus investigate the role of ICOS in cerebral infarction.
MATERIAL AND METHODS: The siRNA of ICOS was first used to suppress the gene expression in cultured lymphocytes. An in vivo study was then performed by intravenous application of ICOS siRNA in cerebral infarction rats. Survival rates, neurological scores, serum tumor necrosis factor (TNF)-α, interleukin (IL)-1, and IL-17 levels were observed.
RESULTS: The expression of ICOS in cultured lymphocytes was significantly suppressed by siRNA. In the in vivo study, the application of siRNA effectively lowered mortality rates of rats, in addition to the improvement of neurological behaviors and amelioration of cerebral tissue damage. Serum levels of TNF-α, IL-1 and IL-17 were all significantly suppressed after siRNA injection.
CONCLUSIONS: ICOS siRNA can protect brain tissues from ischemia injuries after cerebral infarction, improve limb movement and coordination, lower the mortality rate of rats, and inhibit T cell-induced cytokines. These results collectively suggest the potential treatment efficacy of ICOS siRNA against cerebral infarction.

Keywords: cerebral infarction, Hyperlipoproteinemia Type IV, Rats, Inbred BUF

Published: 2015-10-05

Effectiveness of Polyvalent Bacterial Lysate and Autovaccines Against Upper Respiratory Tract Bacterial C...

Olaf Zagólski, Paweł Stręk, Andrzej Kasprowicz, Anna Białecka

Med Sci Monit 2015; 21:2997-3002

DOI: 10.12659/MSM.893779

Published: 2015-10-04

Association Between ESR1 PvuII, XbaI, and P325P Polymorphisms and Breast Cancer Susceptibility: A Meta-An...

Yiming Zhang, Ming Zhang, Xiaosong Yuan, Zhichen Zhang, Ping Zhang, Haojie Chao, Lixia Jiang, Jian Jiang

Med Sci Monit 2015; 21:2986-2996

DOI: 10.12659/MSM.894010