RECK Gene Polymorphism is Associated with Susceptibility and Prognosis of Wilms’ Tumor in Chinese Children
Yang Yu, Yuanjun Hu, Kaisheng Li, Zhihong Chen, Hongmei Zhang, Lei Zhang
(Department of Pediatric Surgery, Jinan Children’s Hospital, Jinan, Shandong, China (mainland))
Med Sci Monit 2015; 21:1928-1933
Wilms’ tumor (WT) is the most common malignant renal tumor in children. Previous studies suggested the reversion-inducing, cysteine-rich protein with Kazal motifs (RECK) down-regulation might have a role in numerous human cancers. The current study was done to investigate the associations of RECK single-nucleotide polymorphisms (SNPs) with the WT susceptibility in Chinese children.
MATERIAL AND METHODS: We analyzed 2 SNPs (rs10972727and rs11788747) in a total of 97 WT children and 194 healthy matched controls (1:2 ratio) by real-time PCR and PCR-RFLP genotyping analysis.
RESULTS: We found that the G allele of rs11788747 in the RECK gene was significantly associated with WT in Chinese children (OR=0.7, 95% CI: 0.45–0.99; P=0.042); as with another SNP rs10972727, however, no statistically significant difference was detected. Further analysis showed there was also a statistically significant difference in genotype frequencies between terminal tumor stage (P=0.026) and metastatic groups (P=0.002).
CONCLUSIONS: The present data indicate that there is a significant association between mutant G of rs11788747 in RECK and WT risk. G carriers with advanced tumor stage or with metastasis might have an increased risk of WT.
Keywords: Genes, Tumor Suppressor, Genes, Wilms Tumor, Nephrology, Polymorphism, Single Nucleotide