Published: 2016-02-09

Antiviral Activity of Hatay Propolis Against Replication of Herpes Simplex Virus Type 1 and Type 2

Ayse Yildirim, Gulay Gulbol Duran, Nizami Duran, Kemal Jenedi, Behiye Sezgin Bolgul, Meral Miraloglu, Mustafa Muz

(Department of Histology and Embryology, Mustafa Kemal University, Medical Faculty, Hatay, Turkey)

Med Sci Monit 2016; 22:422-430

DOI: 10.12659/MSM.897282

BACKGROUND: Propolis is a bee product widely used in folk medicine and possessing many pharmacological properties. In this study we aimed to investigate: i) the antiviral activities of Hatay propolis samples against HSV-1 and HSV-2 in HEp-2 cell line, and ii) the presence of the synergistic effects of propolis with acyclovir against these viruses.
MATERIAL AND METHODS: All experiments were carried out in HEp-2 cell cultures. Proliferation assays were performed in 24-well flat bottom microplates. We inoculated 1x105 cells per ml and RPMI 1640 medium with 10% fetal calf serum into each well. Studies to determine cytotoxic effect were performed. To investigate the presence of antiviral activity of propolis samples, different concentrations of propolis (3200, 1600, 800, 400, 200, 100, 75, 50, and 25 μg/mL) were added into the culture medium. The amplifications of HSV-1 and HSV-2 DNA were performed by real-time PCR method. Acyclovir (Sigma, USA) was chosen as a positive control. Cell morphology was evaluated by scanning electron microscopy (SEM).
RESULTS: The replication of HSV-1 and HSV-2 was significantly suppressed in the presence of 25, 50, and 100 μg/mL of Hatay propolis. We found that propolis began to inhibit HSV-1 replication after 24 h of incubation and propolis activity against HSV-2 was found to start at 48 h following incubation. The activity of propolis against both HSV-1 and HSV-2 was confirmed by a significant decrease in the number of viral copies.
CONCLUSIONS: We determined that Hatay propolis samples have important antiviral effects compared with acyclovir. In particular, the synergy produced by antiviral activity of propo... read more

Keywords: Antiviral Agents, DNA Replication, Herpesvirus 1, Human, Herpesvirus 2, Human, Propolis

Published: 2016-02-08

Diethylstilbestrol Regulates the Expression of LGR8 in Mouse Gubernaculum Testis Cells

Shouxing Duan, Xuewu Jiang, Xuan Zhang, Lei Xie, Zongbo Sun, Shuhua Ma, Jianhong Li

Med Sci Monit 2016; 22:416-421

DOI: 10.12659/MSM.895089

Published: 2016-02-08

Retraction note: Correlation Between High-Density Lipoprotein and Monocyte Subsets in Patients with Stabl...

George B. Stefano

Med Sci Monit 2016; 22:415-415

DOI: 10.12659/MSM.897961