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Published: 2015-05-03

High IL-35 Pleural Expression in Patients with Tuberculous Pleural Effusion

Xuan Dong, Jiong Yang

(Department of Respiratory Medicine, Zhongnan Hospital of Wuhan University No. 169, Wuhan, Hubei, China (mainland))

Med Sci Monit 2015; 21:1261-1268

DOI: 10.12659/MSM.892562


Background: IL-35 is a novel anti-inflammatory and immunosuppressive cytokine primarily produced by Treg cells, and is involved in inflammatory diseases and autoimmune diseases. However, its roles in tuberculous pleural effusion (TPE) remain unknown. We aimed to investigate the potential involvement of IL-35 in TPE.
Material and Methods: Thirty TPE patients and 20 lung cancer patients with malignant pleural effusion (MPE) were recruited. Samples of pleural effusion (100 mL) were collected after traditional pleurocentesis. Blood was sampled from TPE patients. Mononuclear cells were isolated by Ficoll-Hypaque gradient. Proportions of Th1, Th17, and IL-35-producing cells were analyzed by flow cytometry. IL-35 was assessed by real-time RT-PCR, ELISA, and immunofluorescence. An ELISPOT assay was used to assess the effect of IL-35 on pleural effusion mononuclear cells (PEMCs).
Results: Proportions of IL-35-producing cells were higher in TPE compared with MPE (49.4±6.0 vs. 15.8±5.4%, P<0.001) and blood from TPE patients (49.4±6.0% vs. 16.6±3.1, P<0.001). IL-35, IL-17 and IFN-γ were elevated in TPE compared with MPE (all P<0.01). ELISPOT assay showed that IL-35 reduced the proportion of IFN-γ-producing CD4+ T cells in TPE. IL-35 mRNA expression was higher in TPE compared with MPE (P<0.001). Immunofluorescence showed that IL-35-positive cells were present in pleural tissues from TPE patients.
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Keywords: Interleukin-17, Interleukin-18, Interleukins, Natural Killer T-Cells, Pleural Effusion, Tuberculosis




Published: 2015-05-02

Expression of Cold-Inducible RNA-Binding Protein (CIRP) in Pituitary Adenoma and its Relationships with T...


Mingguang Wang, Huan Zhang, Xueyuan Heng, Qi Pang, Aigang Sun

Med Sci Monit 2015; 21:1256-1260

DOI: 10.12659/MSM.893128

Published: 2015-05-01

Vitamin E Intake and Pancreatic Cancer Risk: A Meta-Analysis of Observational Studies


Lujian Peng, Xiangde Liu, Qian Lu, Tengqian Tang, Zhanyu Yang

Med Sci Monit 2015; 21:1249-1255

DOI: 10.12659/MSM.893792