Current Volume 20, 2014
Calreticulin contributes to C1q-dependent recruitment of microglia in the leech Hirudo medicinalis following a CNS injury
Francoise Le Marrec-Croq, Annelise Bocquet-Garcon, Jacopo Vizioli, Christelle Vancamp, Francesco Drago, Julien Franck, Maxence Wisztorski, Michel Salzet, Pierre-Eric Sautiere, Christophe Lefebvre
Med Sci Monit 2014;20:644-653
The medicinal leech is considered as a complementary and appropriate model to study immune functions in the central nervous system (CNS). In a context in which an injured leech’s CNS can naturally restore normal synaptic connections, the accumulation of microglia (immune cells of the CNS that are exclusively resident in leeches) has been shown to be essential at the lesion to engage the axonal sprouting. HmC1q (Hm for Hirudo medicinalis) possesses chemotactic properties that are important in the microglial cell recruitment by recognizing at least a C1q binding protein (HmC1qBP alias gC1qR).
Material and Methods: Recombinant forms of C1q were used in affinity purification and in vitro chemotaxis...
Keywords: Head Injuries, Closed, Complement C1q, Calreticulin, Microglia