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Published: 2015-01-29

Sepsis Induced by Staphylococcus aureus: Participation of Biomarkers in a Murine Model

Thiago Henrique Caldeira de Oliveira, Aline Teixeira Amorim, Izadora Souza Rezende, Maysa Santos Barbosa, Hellen Braga Martins, Anne Karoline Pereira Brito, Ewerton Ferraz Andrade, Gleisy Kelly Neves Gonçalves, Guilherme Barreto Campos, Robson Amaro Augusto Silva, Jorge Timenetsky, Lucas Miranda Marques

(Multidisciplinary Institute of Health, Federal University of Bahia, Vitória da Conquista, Brazil)

Med Sci Monit 2015; 21:345-355

DOI: 10.12659/MSM.892528


Background: This study aimed to evaluate the role of biomarkers in the pathophysiological process induced by a Staphylococcus aureus strain obtained in a hospital environment. For this, we intraperitoneally inoculated groups of male BALB/c mice with S. aureus, using a clinical isolate (CI) of S. aureus.
Material and Methods: Mice were divided into groups according to time of euthanasia (24, 48, 72, 96, 120, 144, and 168 hours of infection). After being euthanized, blood samples were collected for quantification of microorganisms and leukocytes, as well as measurement of biomarkers of tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), C-reactive protein (CRP), and Procalcitonin (PCT) by ELISA. Heart, kidneys, and lungs were removed for histopathological analysis, assessment of biomarkers of tissue expression by RT-PCR (polymerase chain reaction with reverse transcriptase), and quantification of microorganisms by real-time quantitative PCR (real-time PCR).
Results: The animals infected at between 120 hours and 168 hours had the highest blood levels of S. aureus. We observed that infection promoted increases in the levels of circulating neutrophils and monocytes. However, there was a reduction of circulating neutrophils and monocytes after 96 hours of infection. The infected mice also had increased levels of blood lymphocytes. In this model of infection with S. aureus, IL-6, CRP, and PCT demonstrated greater fidelity as markers of infection, since serum levels were elevate... read more

Keywords: Biological Markers, Sepsis, Staphylococcus aureus




Published: 2015-01-28

A New Generation of Biomarkers Tests of Myocardial Necrosis: The Real Quality a Physician can get from th...


Rafał Nikodem Wlazeł, Jarosław Kasprzak, Marek Paradowski

Med Sci Monit 2015; 21:338-344

DOI: 10.12659/MSM.892033

Published: 2015-01-28

Superselective Renal Artery Embolization in the Treatment of Iatrogenic Bleeding into the Urinary Tract


Tomasz Ząbkowski, Piotr Piasecki, Henryk Zieliński, Andrzej Wieczorek, Krzysztof Brzozowski, Piotr Zięcina

Med Sci Monit 2015; 21:333-337

DOI: 10.12659/MSM.892112