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Published: 2016-06-28

Identification of miRNA/mRNA-Negative Regulation Pairs in Nasopharyngeal Carcinoma

Minglei Liu, Kangru Zhu, Xinmei Qian, Wei Li

(Department of Otolaryngology, Head and Neck Surgery, Jining No. 1 People’s Hospital, Jining, Shandong, China (mainland))

Med Sci Monit 2016; 22:2215-2234

DOI: 10.12659/MSM.896047


BACKGROUND: Nasopharyngeal carcinoma (NPC) is a common malignancy in South-East Asia. NPC is characterized by distant metastasis and poor prognosis. The pathophysiological mechanism of nasopharyngeal carcinoma is unknown. This study aimed to identify the crucial miRNAs in nasopharyngeal carcinoma and their target genes, and to discover the potential mechanism of nasopharyngeal carcinoma development.
MATERIAL AND METHODS: Microarray expression profiling of miRNA and mRNA from the Gene Expression Omnibus database was downloaded, and we performed a significance analysis of differential expression. An interaction network of miRNAs and target genes was constructed. The underlying function of differentially expressed genes was predicted through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. To validate the microarray analysis data, significantly different expression levels of miRNAs and target genes were validated by quantitative real-time polymerase chain reaction.
RESULTS: We identified 27 differentially expressed miRNAs and 982 differentially expressed mRNAs between NPC and normal control tissues. 12 miRNAs and 547 mRNAs were up-regulated and 15 miRNAs and 435 mRNAs were down-regulated in NPC samples. We found a total of 1185 negative correlation pairs between miRNA and mRNA. Differentially expressed target genes were significantly enriched in pathways in cancer, cell cycle, and cytokine-cytokine receptor interaction signaling pathways. Significantly differentially expressed miRNAs and genes, such as hsa-miR-205, hsa-miR-18b, hsa-miR-632, hsa-miR-130a, hsa-miR-34b, PIGR, SMPD3, CD22, DTX4, and CDC6, may play essent... read more

Keywords: Gene Regulatory Networks, MicroRNAs, Nasopharyngeal Neoplasms, Real-Time Polymerase Chain Reaction



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