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Published: 2016-05-29

MicroRNA-208b Alleviates Post-Infarction Myocardial Fibrosis in a Rat Model by Inhibiting GATA4

Chaoyuan Zhou, Qintao Cui, Guobao Su, Xiaoliang Guo, Xiaochen Liu, Jie Zhang

(Department of Cardiovascular Surgery, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan, China (mainland))

Med Sci Monit 2016; 22:1808-1816

DOI: 10.12659/MSM.896428


BACKGROUND: Myocardial infarction affects the health of many people. Post-infarction myocardial fibrosis has attracted much attention, but details of the mechanism remain elusive. In this study, the role of microRNA-208b (miR-208b) in modulating post-infarction myocardial fibrosis and the related mechanism were investigated.
MATERIAL AND METHODS: A rat model of myocardial infarction induced by ligating the left anterior descending artery was used to analyze the expression and roles of miR-208b by overexpression with the lentivirus vector of pre-miR-208b. Myocardial function was assessed and the expression of fibrosis-related factors type I collagen (COL1) and ACTA2 (alias αSMA) was detected. Myocardial fibroblasts isolated from newborn rats were transfected with luciferase reporter vectors containing wild-type or mutant Gata4 3’ UTR to verify the relationship between Gata4 and miR-208b. We then transfected the specific small interference RNA of Gata4 to detect changes in COL1 and ACTA2.
RESULTS: miR-208b was down-regulated in hearts of model rats (P<0.01). Overexpressing miR-208b improved myocardial functions, such as reducing the infarction area (P<0.05) and promoting LVEF and LVFS (P<0.01), and inhibited COL1 and ACTA2 (P<0.01). Luciferase reporter assay proved Gata4 to be the direct target of miR-208b, with the target s... read more

Keywords: Collagen Type I, Fibrosis, GATA4 Transcription Factor, MicroRNAs, Myocardial Infarction




Published: 2016-05-28

Biofilm-Related Genes: Analyses in Multi-Antibiotic Resistant Acinetobacter Baumannii Isolates From Mainl...


Hui Liu, Yong-Quan Wu, Li-Ping Chen, Xiang Gao, Hao-Nan Huang, Fu-Lan Qiu, Ding-Chang Wu

Med Sci Monit 2016; 22:1801-1807

DOI: 10.12659/MSM.898959

Published: 2016-05-27

High-Intensity Chemotherapy is Associated with Better Prognosis in Young Patients with High-Risk Diffuse ...


Xiaorong Ma, Yan Xu, Wanggang Zhang, Jin Wang, Xingmei Cao, Yinxia Chen, Aili He, Jie Liu, Jianli Wang, Wanhong Zhao, Yun Yang

Med Sci Monit 2016; 22:1792-1800

DOI: 10.12659/MSM.895383