eISSN: 1643-3750


Get your full text copy in PDF

Absorption rates and free radical scavenging values of vitamin C-lipid metabolites in human lymphoblastic cells

Benjamin S Weeks, Pedro P Perez

Med Sci Monit 2007; 13(10): BR205-210

ID: 502324

Background: In this study we investigated the cellular absorption rates, antioxidant and free radical scavenging activity of vitamin C-lipid metabolites. The absorption was measured in a human lymphoblastic cell line using a spectrophotometric technique.
Material/Methods: Cellular vitamin C levels in the human lymphoblastic H9 cell line were measured using the 2,4-dinitrophenylhydrazine spectrophotometric technique. Free radical scavenging activity of vitamin C-lipid metabolites was measured by the reduction of 1,1-diphenyl-2-picryl hydrazyl (DPPH) to 1,1-diphenyl-2-picryl hydrazine. Vitamin C-lipid metabolite scavenging of peroxyl radical oxygen reactive species (ORAC) was determined by fluorescence spectrophotometry.
Results: Compared to ascorbic acid (AA), calcium ascorbate (CaA), and calcium ascorbate-calcium threonate-dehydroascorbate (Ester-C®), vitamin C-lipid metabolites (PureWay-C™) were more rapidly absorbed by the H9 human T-lymphocytes. The vitamin C-lipid metabolites (PureWay-C™) also reduced pesticide-induced T-lymphocyte aggregation by 84%, while calcium ascorbate-calcium threonate-dehydroascorbate (Ester-C®) reduced aggregation by only 34%. The vitamin C-lipid metabolites (PureWay-C™) demonstrated free radical scavenging activity of nearly 100% reduction of DPPH at 20 µg/ml and oxygen radical scavenging of over 1200µ Trolox® equivalents per gram.
Conclusions:    These data demonstrate that the vitamin C-lipid metabolites (PureWay-C™) are more rapidly taken-up and absorbed by cells than other forms of vitamin C, including Ester-C®. This increased rate of absorption correlates with an increased protection of the T-lymphocytes from pesticide toxicities. Further, vitamin C-lipid metabolites (PureWay-C™) are a potent antioxidant and have significant free radical scavenging capabilities.

This paper has been published under Creative Common Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) allowing to download articles and share them with others as long as they credit the authors and the publisher, but without permission to change them in any way or use them commercially.
I agree