03 March 1997
Implementation of an image analysis system for morphological description of skin mast cells in urticaria pigmentosa
Anna Zalewska, Michał Strzelecki, Jacek SygutMed Sci Monit 1997; 3(2): MT260-265 :: ID: 500736
Abstract
The aim of the study was to develop a new method to describe the morphology and localization of mast cells in urticaria pigmentosa and healthy skin. We employed Astra blue stain to visualize tissue mast cells with subsequent unique identification of those cells within the whole picture. The procedure consisted of the following steps: 1. Astra blue staining of the mast cells allowed us to threshold the image and as a result mast cell objects were obtained marked in black on a white background. 2. Geometrical features such as area, edge and maximal diameter of the mast cell objects were calculated, 3. The dermoepidermal junction was considered as a texture and for this texture a set of statistical features was calculated; image segmentation resulted in an image region with a clearly separated dermoepidermal junction. 4. The Euclidian distance was calculated from the mast cell and the dermoepidermal junction.Astra blue stain allowed the differentiation between mast cells and the surrounding tissue elements. The employed methods of image analysis allowed quick, automated characterization of skin mast cells. In conclusion, it should be pointed out that the employed image analysis techniques may be of value in describing localization and morphological parameters of mast cells. Refinement of the method may be useful to image other cell types as well.
Keywords: mast cell, image analysis, morphology
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
06 Mar 2024 : Clinical Research
Prevalence and Variation of Medical Comorbidities in Oral Surgery Patients: A Retrospective Study at Jazan ...Med Sci Monit In Press; DOI: 10.12659/MSM.943884
08 Mar 2024 : Clinical Research
Evaluation of Foot Structure in Preschool Children Based on Body MassMed Sci Monit In Press; DOI: 10.12659/MSM.943765
15 Apr 2024 : Laboratory Research
The Role of Copper-Induced M2 Macrophage Polarization in Protecting Cartilage Matrix in OsteoarthritisMed Sci Monit In Press; DOI: 10.12659/MSM.943738
07 Mar 2024 : Clinical Research
Knowledge of and Attitudes Toward Clinical Trials: A Questionnaire-Based Study of 179 Male Third- and Fourt...Med Sci Monit In Press; DOI: 10.12659/MSM.943468
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952