01 February 2006
Bifenthrin inhibits neurite outgrowth in differentiating PC12 cells.
Van Tran, Natalie Hoffman, Adaobi Mofunanaya, Stephen Pryor, Olutosin Ojugbele, Ashlea McLaughlin, Lydia Gibson, Josephine A. Bonventre, Katherine Flynn, Benjamin WeeksMed Sci Monit 2006; 12(2): BR57-62 :: ID: 445228
Abstract
Background: Bifenthrin is a third generation member of the synthetic pyrethroidfamily of insecticides. As a new pesticide within a relatively new class of pesticides, bifenthrin isconsidered relatively safe. Here, we used the PC12 neuronal cell line to examine the effect of bifenthrinon the formation of neurites and the potential developmental neurotoxicity of this pesticide. Material/Methods:PC12 cells were exposed to varying concentrations of technical grade bifenthrin or Ortho Home Defense[sup]®[/sup].Cell viability was determined using the AlmarBlue Toxicity Assay. Nontoxic concentrations of these chemicalswere concomitantly with nerve growth factor and neurite outgrowth was assessed. Results: Ortho Home Defense[sup]®[/sup]preparation reduced PC12 cell viability by approximately 50% and 70% at dilutions that correlate to bifenthrinconcentrations of 10[sup]-5[/sup] M and 10[sup]®[/sup] M, respectively. In contrast, technical grade bifenthrin, was nottoxic to PC12 cells at 10[sup]-3[/sup] M, which was the highest concentration tested that was soluble. At "nontoxic"concentrations of 10[sup]-7[/sup] M and 10[sup]-6[/sup] M, the Ortho Home Defense[sup]®[/sup] inhibited nerve growth factor-mediatedneurite outgrowth by 30% and 55% respectively. Furthermore the nontoxic concentrations of technical gradebifenthrin of 10[sup]-6[/sup] M and 10[sup]-3[/sup] M inhibited neurite outgrowth by approximately 35% and 75% respectively.Conclusions: These data suggest that the toxicity of the Ortho Home Defense[sup]®[/sup] preparation was dueto the "inert" additives in the preparation and not the bifenthrin itself. Further, these data suggestthat, even in the absence of overt toxicity, bifenthrin may have deleterious effects to developing nervoussystem.
Keywords: Cell Survival - drug effects, Insecticides - toxicity, Neurites - pathology, Neurotoxins - toxicity, Pyrethrins - toxicity
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
15 Apr 2024 : Laboratory Research
The Role of Copper-Induced M2 Macrophage Polarization in Protecting Cartilage Matrix in OsteoarthritisMed Sci Monit In Press; DOI: 10.12659/MSM.943738
07 Mar 2024 : Clinical Research
Knowledge of and Attitudes Toward Clinical Trials: A Questionnaire-Based Study of 179 Male Third- and Fourt...Med Sci Monit In Press; DOI: 10.12659/MSM.943468
08 Mar 2024 : Animal Research
Modification of Experimental Model of Necrotizing Enterocolitis (NEC) in Rat Pups by Single Exposure to Hyp...Med Sci Monit In Press; DOI: 10.12659/MSM.943443
18 Apr 2024 : Clinical Research
Comparative Analysis of Open and Closed Sphincterotomy for the Treatment of Chronic Anal Fissure: Safety an...Med Sci Monit In Press; DOI: 10.12659/MSM.944127
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952