Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 January 2000

Plasma HDL-cholesterol has an effect on nitric oxide production and arachidonic acid metabolism in the platelet membranes of coronary heart disease patients without LDL-hypercholesterolemia

Peteris Tretjakovs, Uldis Kalnins, Inese Dabina, Iveta Dinne, Andrejs Erglis, Indulis Kumsars, Antra Jurka

Med Sci Monit 2000; 6(3): CR507-511 :: ID: 421317

Abstract

AIM: To evaluate nitric oxide (NO) production and [3H]arachidonic acid(AA) incorporation into platelet membranes of coronary artery disease (CAD) patients with/without HDL-hypocholesterolemia.MATERIAL: 16 healthy controls (C), 14 CAD patients with plasma HDL-hypocholesterolemia (nCAD) and 14--withoutHDL-hypocholesterolemia (nCAD). All subjects were without peripheral vascular disease and hypertension.The groups were matched for age, sex, BMI. The diagnosis of CAD was substantiated by coronary angiography.METHODS: Nitric oxide end products xNO (NO2- plus NO3-) levels in the platelet membranes were measuredusing anion-exchange chromatography. [3H]AA release from labelled platelets was studied by the methodof Neufeld and Majerus; radioactivity was measured by liquid scintillation counting. Levels of plasmaHDL-cholesterol (HDL-Ch) and triglycerides were enzymatically determined. RESULTS: Significant increase(mean +/- SD; Mann-Whitney U test) of [3H]AA incorporation into platelet membrane phospholipids was notedin CAD patients in comparison with healthy subjects (p < 0.001). A correlation (multiple regression analysis)was established between HDL-C level and [3H]AA (r = -0.58, p < 0.05, n = 28); and between HDL-Ch andNOx levels (r = 0.76, p < 0.05, n = 28) in CAD patients. CAD patients had lower NOx than healthy subjects(p < 0.0001), NOx was lower in the group with decreased HDL-Ch concentration (wCAD 36 +/- 5 vs. nCAD42.3 +/- 6 mumol/mg, p < 0.002). CONCLUSIONS: CAD patients show decreased ability to produce platelet-derivedNO that leads to higher platelet sensitivity to aggregating stimuli. Decreased plasma HDL-Ch may affectAA metabolism and NO production in the platelet membranes of CAD patients without LDL-hypercholesterolemia.

Keywords: Arachidonic Acid, Blood Platelets, Cell Membrane, Comparative Study, Coronary Disease, Hypercholesterolemia, Lipoproteins, HDL Cholesterol, Lipoproteins, LDL Cholesterol, Membrane Lipids, Nitric Oxide, Nitrogen Oxides, Phospholipids, Reference Values, Regression Analysis, Tritium

Add Comment 0 Comments

Editorial

01 March 2024 : Editorial  

Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-Thalassemia

Dinah V. Parums

DOI: 10.12659/MSM.944204

Med Sci Monit 2024; 30:e944204

0:00

In Press

18 Mar 2024 : Clinical Research  

Sexual Dysfunction in Women After Tibial Fracture: A Retrospective Comparative Study

Med Sci Monit In Press; DOI: 10.12659/MSM.944136  

0:00

21 Feb 2024 : Clinical Research  

Potential Value of HSP90α in Prognosis of Triple-Negative Breast Cancer

Med Sci Monit In Press; DOI: 10.12659/MSM.943049  

22 Feb 2024 : Review article  

Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943168  

23 Feb 2024 : Clinical Research  

A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943732  

Most Viewed Current Articles

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750