01 January 2002
Digital image analysis system for the quantification of infiltrates and celladhesion molecules in inflammatory cardiomyopathy.
Michel Noutsias, Matthias Pauschinger, Karsten Ostermann, Felicitas Escher, Jan-Henrik Blohm, Heinz Schultheiss, Uwe KühlMed Sci Monit 2002; 8(5): MT59-71 :: ID: 420831
Abstract
BACKGROUND: We attempted to develop a digital image analysis (DIA) systemfor endomyocardial biopsies (EMBs) to reliably quantify a) biopsy quality, b) immunohistochemically-markedinfiltrates, and c) cell adhesion molecules (CAMs) in relation to net heart area (HA) for the semi-automateddiagnosis of inflammatory cardiomyopathy (InfCM). MATERIAL/METHODS: 140 EMBs from dilated cardiomyopathy(DCM) patients and 14 autopsy heart samples (controls) were immunostained for T-lymphocytes (CD2, CD3,CD4, CD8), beta(2)-integrin+ infiltrates (CD18, LFA-1, Mac-1) and CAMs (immunoglobulin superfamily: ICAM-1,HLA class I, HLA DR, VCAM-1, CD58; selectins: CD62E and CD62P; and the beta(1)-integrin chain CD29).EMB quality was assessed visually on a three-point scale. Infiltrates were quantified visually (per hpf)and by DIA (per mm2 HA). CAM expression was evaluated semiquantitatively and by DIA (area fraction [AF]:stained area relative to HA). RESULTS: DIA-evaluated HA correlated significantly with the visual assessmentof EMB quality. The visual evaluation of both infiltrates and CAMs correlated significantly with therespective DIA-based quantification. DIA-quantified CAM-AF and infiltrates were discriminated by theCAM classification (CAMs+: n=87; 62%) compared to controls. DIA-quantified CAM immunoreactivity correlatedsignificantly with the DIA-quantified counter-receptor+ infiltrates. DIA evaluation of biopsy quality,infiltrates, and CAMs was devoid of inter- and intraobserver variability. CONCLUSIONS: The DIA systempresented here enables standardized and observer-independent assessment of EMB quality and intramyocardialinflammation (density of infiltrates and CAM expression) in DCM biopsies related to HA. Our data confirmthat endothelial CAM count and counter-receptor+ immunocompetent infiltration are interdependent pathogenicand diagnostic hallmarks of InfCM.
Keywords: Antigens, CD3, Biopsy, Cardiomyopathies, Cell Adhesion, Image Processing, Computer-Assisted, Myocardium, Phenotype, Research Support, Non-U.S. Gov
Editorial
01 March 2024 : Editorial
Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-ThalassemiaDOI: 10.12659/MSM.944204
Med Sci Monit 2024; 30:e944204
In Press
18 Mar 2024 : Clinical Research
Sexual Dysfunction in Women After Tibial Fracture: A Retrospective Comparative StudyMed Sci Monit In Press; DOI: 10.12659/MSM.944136
21 Feb 2024 : Clinical Research
Potential Value of HSP90α in Prognosis of Triple-Negative Breast CancerMed Sci Monit In Press; DOI: 10.12659/MSM.943049
22 Feb 2024 : Review article
Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...Med Sci Monit In Press; DOI: 10.12659/MSM.943168
23 Feb 2024 : Clinical Research
A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...Med Sci Monit In Press; DOI: 10.12659/MSM.943732
Most Viewed Current Articles
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952