Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

01 August 2005

Expression regulation of MAO isoforms in monocytic cells in response to Th2cytokines.

Pavlos Chaitidis, Ellen Billett, Ralf J Kuban, Ute Ungethuem, Hartmut Kuhn

Med Sci Monit 2005; 11(8): BR259-265 :: ID: 199709

Abstract

Background: Th2-cytokines, such as interleukins-4 and -13 (IL-4, IL-13),have been identified as alternative stimuli of monocytes/macrophages. We have recently profiled the gene-expressionpattern of IL-4-teated human peripheral monocytes and found that 15-lipoxygenase-1 (15-LOX1) and monoamineoxidase A (MAO-A) are among the five most strongly upregulated gene products in IL-4-treated cells. Transfectionof monocytic cells (U937) with 15-LOX1 also induced MAO-A expression. These data suggested that 15-LOX1products might play a role in the IL4-induced signaling cascade leading to expression of MAO-A in humanmonocytes. Material/Methods: To test this hypothesis we incubated wild-type and 15-LOX1-transfected U937cells with different concentrations of either IL-4 or 15-LOX-products [13S-H(p)ODE, 15S-H(p)ETE] andquantified the expression of 15-LOX1, MAO-A, and MAO-B by activity assays and real-time RT-PCR. Results:Wild-type U937 cells express neither MAO-A nor MAO-B, but after three days of IL4 treatment, MAO-A mRNAwas detected. A similar isoform-specific expression of MAO-A mRNA was observed when U937 cells were transfectedwith 15-LOX1 or when the cells were incubated with primary 15-LOX1 products (hydroperoxy fatty acids)or H2O2. In contrast, the corresponding hydroxy fatty acids were ineffective. Conclusions: These dataindicate that increased intracellular peroxide concentrations (oxidative stress) induce MAO-A expressionin monocytes/macrophages, which normally do not express the enzyme. Our findings also suggest that IL-4-inducedupregulation of MAO-A expression in human peripheral monocytes may proceed via 15-LOX1-dependent and15-LOX1-independent pathways. The biological role of MAO-A expression for monocyte function is discussed.

Keywords: Gene Expression Regulation, Enzymologic - genetics, Arachidonate 15-Lipoxygenase - metabolism, Gene Expression Regulation, Enzymologic - genetics, Interleukin-4 - pharmacology, Isoenzymes - metabolism, Linoleic Acid - metabolism, Monoamine Oxidase - metabolism, Monocytes - enzymology, Th2 Cells - metabolism

Add Comment 0 Comments

Editorial

01 March 2024 : Editorial  

Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-Thalassemia

Dinah V. Parums

DOI: 10.12659/MSM.944204

Med Sci Monit 2024; 30:e944204

0:00

In Press

18 Mar 2024 : Clinical Research  

Sexual Dysfunction in Women After Tibial Fracture: A Retrospective Comparative Study

Med Sci Monit In Press; DOI: 10.12659/MSM.944136  

0:00

21 Feb 2024 : Clinical Research  

Potential Value of HSP90α in Prognosis of Triple-Negative Breast Cancer

Med Sci Monit In Press; DOI: 10.12659/MSM.943049  

22 Feb 2024 : Review article  

Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943168  

23 Feb 2024 : Clinical Research  

A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943732  

Most Viewed Current Articles

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750