01 August 2004
Growth hormone enhances gastric ulcer healing in rats
Stefan Beckert, Nicole Class, Farshid Farrahi, Stephan CoerperMed Sci Monit 2004; 10(8): BR255-258 :: ID: 11740
Abstract
Background: Gastric ulcer healing requires the reconstitution of epithelial structures and underlying connective tissue through cellular proliferation, migration, and differentiation. The systemic application of growth hormone (GH) has shown anabolic effects in postoperative and burn therapy by increasing protein synthesis and attenuating protein catabolism. There is also evidence that GH stimulates cell proliferation and differentiation. In this study we evaluated the impact of GH on gastric ulcer healing.
Material/Methods: Gastric ulcers were induced with a cryoprobe in male Wistar rats (285±11 g). The first group of rodents (n=10) received a daily subcutaneous dose of 2.5 mg/kg growth hormone for seven days. The second group (n=10) received only vehicle. After 7 days, ulcer size was determined photoplanimetrically. Cell proliferation and new vessel growth in the ulcer margin were evaluated by quantitative immunohistochemical staining for proliferating cell nuclear antigen (PCNA) and smooth muscle actin (SMA), respectively.
Results: The systemic application of GH caused a significant increase in body weight (332±7 g vs. 289±13 g; p=0.007). Ulcer size was also reduced significantly compared with controls (5.6±0.8 mm2 vs. 9.9±1 mm2; p=0.005). Immunohistochemical analysis revealed a significant increase in cell proliferation (79.7±0.9% pos. cells vs. 64.7±1.9% pos. cells; p=0.0001) as shown by PCNA expression, and a significant increase in new vessel growth as demonstrated by SMA expression (1762±124 cells/mm2 vs. 1067±77 cells/mm2; p=0.0001).
Conclusions: Growth hormone accelerates gastric ulcer healing by stimulating cell proliferation and angiogenesis.
Keywords: Cell Division - drug effects, Gastric Mucosa - drug effects, Gastric Mucosa - pathology, Growth Hormone - pharmacology, Neovascularization, Pathologic - drug therapy, Rats, Wistar, Stomach Ulcer - drug therapy, Wound Healing - drug effects, Cell Division - drug effects, Gastric Mucosa - pathology, Growth Hormone - pharmacology, Neovascularization, Pathologic - drug therapy, Rats, Wistar, Stomach Ulcer - drug therapy, Wound Healing - drug effects
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
07 Mar 2024 : Clinical Research
Knowledge of and Attitudes Toward Clinical Trials: A Questionnaire-Based Study of 179 Male Third- and Fourt...Med Sci Monit In Press; DOI: 10.12659/MSM.943468
08 Mar 2024 : Animal Research
Modification of Experimental Model of Necrotizing Enterocolitis (NEC) in Rat Pups by Single Exposure to Hyp...Med Sci Monit In Press; DOI: 10.12659/MSM.943443
18 Apr 2024 : Clinical Research
Comparative Analysis of Open and Closed Sphincterotomy for the Treatment of Chronic Anal Fissure: Safety an...Med Sci Monit In Press; DOI: 10.12659/MSM.944127
08 Mar 2024 : Laboratory Research
Evaluation of Retentive Strength of 50 Endodontically-Treated Single-Rooted Mandibular Second Premolars Res...Med Sci Monit In Press; DOI: 10.12659/MSM.944110
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952