Logo Medical Science Monitor

Call: +1.631.470.9640
Mon - Fri 10:00 am - 02:00 pm EST

Contact Us

Logo Medical Science Monitor Logo Medical Science Monitor Logo Medical Science Monitor

06 January 2004

Lymphocytic gastritis in pediatric celiac disease – immunohistochemical study of the intraepithelial lymphocytic component

Ricardo Drut, Rosa Mónica Drut

Med Sci Monit 2004; 10(1): CR38-42 :: ID: 11553

Abstract

Background:Lymphocytic gastritis (LG) is defined by the recognition of >25 intraepithelial lymphocytes (IEL) per 100 surface epithelial cells. Approximately 50% of children with celiac disease (CD) present LG, which mainly involves the gastric antrum and disappears after a gluten-free diet. This intraepithelial population of lymphocytes has not been immunophenotypically characterized for this age group.Material/Methods: We immunohistochemically analyzed 5 formalin-fixed, paraffin embedded biopsies of LG in children with untreated celiac disease using a panel of antibodies. All patients were diagnosed on the bases of clinical, laboratory and histopathological data.Results: All showed similar results. IEL proved to be CD45RO, CD3, CD7, CD8, and Tia-1 positive, while CD4, CD20, CD79a, CD56, CD57, granzyme B, perforin, TCR gamma-delta, TCR beta (V-19), CD95 (FAS), CD95-L (FAS-L) and HLA II were negative. Many IEL were in apoptosis. CD45RO, CD3 and CD7 presented as membranous staining, while Tia-1 resulted in intracytoplasmic granular brown dots. In 3 cases there were rare lymphocytes with granzyme B-positive granules localized to the lamina propria. None presented Helicobacter pylori -like organisms at the luminal surface.Conclusions: LG associated with CD in children contains a peculiar CD8+ intraepithelial T-lymphocyte population which immunohistochemically lacks perforin and granzyme B, undergoes apoptosis, and is not associated with substantial damage to the epithelial cells. Our results fit with those reported in adults except for the negative results for granzyme B. The findings appear disparate from the ones described in the duodeno-jejunal mucosa in untreated celiac disease.

Keywords: Adolescent, Antigens - metabolism, Antigens, CD45 - metabolism, CD8-Positive T-Lymphocytes - immunology, CD8-Positive T-Lymphocytes - pathology, Celiac Disease - immunology, Celiac Disease - pathology, Child, Child, Preschool, Epithelium - immunology, Epithelium - pathology, Gastric Mucosa - immunology, Gastric Mucosa - pathology, Gastritis - immunology, Gastritis - pathology, Lymphocyte Subsets - immunology, Lymphocyte Subsets - pathology, Adolescent, Antigens - metabolism, Antigens, CD45 - metabolism, CD8-Positive T-Lymphocytes - pathology, Celiac Disease - pathology, Child, Child, Preschool, Epithelium - pathology, Gastric Mucosa - pathology, Gastritis - pathology, Lymphocyte Subsets - pathology

Add Comment 0 Comments

Editorial

01 March 2024 : Editorial  

Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-Thalassemia

Dinah V. Parums

DOI: 10.12659/MSM.944204

Med Sci Monit 2024; 30:e944204

0:00

In Press

18 Mar 2024 : Clinical Research  

Sexual Dysfunction in Women After Tibial Fracture: A Retrospective Comparative Study

Med Sci Monit In Press; DOI: 10.12659/MSM.944136  

0:00

21 Feb 2024 : Clinical Research  

Potential Value of HSP90α in Prognosis of Triple-Negative Breast Cancer

Med Sci Monit In Press; DOI: 10.12659/MSM.943049  

22 Feb 2024 : Review article  

Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943168  

23 Feb 2024 : Clinical Research  

A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...

Med Sci Monit In Press; DOI: 10.12659/MSM.943732  

Most Viewed Current Articles

16 May 2023 : Clinical Research  

Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...

DOI :10.12659/MSM.940387

Med Sci Monit 2023; 29:e940387

0:00

17 Jan 2024 : Review article  

Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron Variant

DOI :10.12659/MSM.942799

Med Sci Monit 2024; 30:e942799

0:00

14 Dec 2022 : Clinical Research  

Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase Levels

DOI :10.12659/MSM.937990

Med Sci Monit 2022; 28:e937990

0:00

01 Jan 2022 : Editorial  

Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...

DOI :10.12659/MSM.935952

Med Sci Monit 2022; 28:e935952

0:00

Your Privacy

We use cookies to ensure the functionality of our website, to personalize content and advertising, to provide social media features, and to analyze our traffic. If you allow us to do so, we also inform our social media, advertising and analysis partners about your use of our website, You can decise for yourself which categories you you want to deny or allow. Please note that based on your settings not all functionalities of the site are available. View our privacy policy.

Medical Science Monitor eISSN: 1643-3750
Medical Science Monitor eISSN: 1643-3750