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Boschniakia Rossica Polysaccharide Triggers Laryngeal Carcinoma Cell Apoptosis by Regulating Expression of Bcl-2, Caspase-3, and P53

Chunping Yao, Xiujuan Cao, Zheng Fu, Jing Tian, Wei Dong, Jin Xu, Kang An, Limin Zhai, Jinming Yu

(Department of Radiation Oncology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China (mainland))

Med Sci Monit 2017; 23:2059-2064

DOI: 10.12659/MSM.901381

Published: 2017-04-29

BACKGROUND: Laryngeal cancer is a malignant head and neck tumor with high morbidity and high mortality in humans. Recently, treatments with Chinese medicines and their extracts have gradually received great attention, and studies suggest that Boschniakia rossica polysaccharide (BRP) has potential anti-tumor activity. Therefore, this study investigating the role of BRP in inducing apoptosis in human laryngeal carcinoma cells.
MATERIAL AND METHODS: The BRP was extracted with organic solvent and HR column. We treated Hep2 laryngeal carcinoma cells with different concentrations of BRP, then assessed cell growth inhibition rate by flow cytometry and apoptosis index by TUNEL staining. The protein expression of p53, Bcl-2, Bax, and caspase-3 were analyzed by Western blot.
RESULTS: Flow cytometry results showed that BRP inhibited Hep2 cell proliferation in a dose-dependent manner (p<0.05), and TUNEL staining indicated that BRP significantly increased Hep2 apoptosis index (p<0.05). Western blot results showed that the expression levels of p53 and activation of caspase-3 in Hep2 cells were significantly up-regulated (p<0.05), while the expression of Bcl-2 was significantly down-regulated (p<0.05).
CONCLUSIONS: BRP might induce cell apoptosis by regulating the expression level of cell apoptosis-associated proteins, suggesting strong anti-laryngeal cancer activity.

Keywords: Apoptosis, Caspase 3, Genes, bcl-2, Genes, p53, Laryngeal Neoplasms