26 January 2017 : Laboratory Research
Knockdown of TCTN1 Strongly Decreases Growth of Human Colon Cancer Cells
Xiaoyu Dai1ACEG*, Mingjun Dong1BDF, Hua Yu1BDF, Yangyang Xie1BDF, Yongming Yu1DF, Yisheng Cao1C, Zhenfang Kong1CF, Baofeng Zhou1BD, Yidong Xu1DG, Tong Yang1DG, Keqiang Li2AEGDOI: 10.12659/MSM.899595
Med Sci Monit 2017; 23:452-461
Abstract
BACKGROUND: Tectonic family member 1 (TCTN1), a member of the tectonic family, is involved in several developmental processes and is aberrantly expressed in multiple solid tumors. However, the expression and regulation of TCTN1 in human colorectal cancer (CRC) is still not clear.
MATERIAL AND METHODS: The expression of TCTN1 mRNA was first explored by using Oncomine microarray datasets. TCTN1 expression was silenced in human CRC cell lines HCT116 and SW1116 via RNA interference (RNAi). Furthermore, we investigated the effect of TCTN1 depletion on CRC cell growth by MTT, colony formation, and flow cytometry in vitro.
RESULTS: In this study, meta-analysis showed that the expressions of TCTN1 mRNA in CRC specimens were significantly higher than that in normal specimens. Knockdown of TCTN1 expression potently inhibited the abilities of cell proliferation and colony formation as determined. Flow cytometry analysis showed that depletion of TCTN1 could cause cell cycle arrest at the G2/M phase. In addition, Annexin V/7-AAD double-staining indicated that TCTN1 silencing promoted cell apoptosis through down-regulation of caspase 3 and Bcl-2 and upregulation of cleaved caspase 3 and PARP.
CONCLUSIONS: Our results indicate that TCTN1 may be crucial for CRC cell growth, providing a novel alternative to target therapies of CRC. Further research on this topic is warranted.
Keywords: Genes, Neoplasm, RNA, Small Interfering
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