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eISSN: 1643-3750

MALDI imaging mass spectrometry in ovarian cancer for tracking, identifying, and validating biomarkers

Mohamed El Ayed, David Bonnel, Remi Longuespée, Céline Castelier, Julien Franck, Daniele Vergara, Annie Desmons, Aurélie Tasiemski, Abderraouf Kenani, Denis Vinatier, Robert Day, Isabelle Fournier, Michel Salzet

Med Sci Monit 2010; 16(8): BR233-245

ID: 881095

Published: 2010-08-01

Background:    Among biomarkers, cancer-antigen 125 (CA-125) is the most studied. We propose an analytical tool to track ovarian carcinoma biomarkers, that is, the MALDI mass spectrometry imaging.
    Material/Methods:    Ovarian carcinomas and benign ovaries were directly analyzed by MALDI-TOF-MS. After automatic profiling and mass spectrometry imaging analyses, hierarchical clustering based on principal component analysis in nonsupervised mode was carried out. On the same samples, preparations were performed to investigate peptides, then proteins, followed by high mass proteins, in an automatic profiling to specific signatures for diagnosis. Using tissue bottom-up strategy on tissue digestion, and mass spectrometry imaging after by shotgun sequencing by nalano-LC-IT-MS in MS/MS mode from washing samples from on tissue digested peptides, several biomarkers were found.
    Results:    A list of specific biomarkers from the ovarian carcinoma regions was obtained and classified as proteins associated with cell proliferation, involved in immune response modulation, signaling to the cytoskeleton, and tumor progression. These specific biomarkers were then validated by immunocytochemistry using Tag-mass technology, cell biology, Western blot, and by PCR (using SKOV-3 ovarian epithelial cancer cells). A link between the immune regulation (innate immunity, tolerance) and virus cause is also discussed.
    Conclusions:    From the biomarkers identified, proteins involved in immune response modulation and cell proliferation have been pointed out in this study. Two new markers have been identified using such a strategy, that is, fragment C-terminal of the PSME1 (Reg-Alpha) and mucin-9.

Keywords: Principal Component Analysis, Ovarian Neoplasms - pathology, Neoplasm Staging, Neoplasm Proteins - metabolism, Nanotechnology, Molecular Weight, Molecular Sequence Data, Humans, Gene Expression Regulation, Neoplastic, Amino Acid Sequence, Female, Adenocarcinoma, Mucinous, Reproducibility of Results, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods, Tumor Markers, Biological - metabolism