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Differential neuronal expression of manganese superoxide dismutase in Alzheimer'sDisease.

David L. Marcus, James A. Strafaci, Michael L. Freedman

Med Sci Monit 2006; 12(1): BR8-14

ID: 443169

Published: 2005-12-22


Background: Manganese-dependent superoxide dismutase (MnSOD) is a majordefense mechanism against potential cellular damage by reactive oxygen species (ROS). We have reportedincreased lipid peroxidation and decreased Cu/Zn SOD enzymatic activity in the temporal cortex of Alzheimer'sdiseased (AD) brains. Material/Methods: We now report the expression of MnSOD in the hippocampus of AD(n=8) and non-AD patients (n=7) via immunohistochemical methods, in both neuronal and non neuronal cells.We tested the hypothesis that there is differential expression of MnSOD in the CA1, CA2/3, and CA4 regionof the hippocampus which may account for the neuronal loss seen in Alzheimer's disease. For neuronalcells, profile counts were made and expressed as positive neuronal profiles (MnSOD-IR) per mm(2) withinhippocampal regions CA1, CA2/3, and CA4. Results: The AD MnSOD-IR counts were over 9-fold higher in theCA2/3 region (p

Keywords: Alzheimer Disease - enzymology, Regression Analysis, Neurons - enzymology, Middle Aged, Male, Immunohistochemistry, Humans, Hippocampus - enzymology, Free Radicals - metabolism, Female, Animals, Aged, Aged, 80 and over, Superoxide Dismutase - metabolism



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