15 May 2002
The rate of loss of eyelid reflex following thiopental administration in hypo-and hypergonadism in rabbits.
Beata Badowicz, Andrzej Starczewski, Bogdan Szymański, Leon Drobnik, Cezary PakulskiMed Sci Monit 2002; 8(5): BR179-183 :: ID: 420848
Abstract
BACKGROUND: The aim of this research was to explain whether different hormonalconditions caused by disturbed concentrations of estrogens and progesterone might lead to alterationof CNS reaction following administration of the hypnotic agent thiopental. The investigated factor wasthe rate of loss of the eyelid reflex after intravenous thiopental administration, since this correspondswith loss of consciousness. MATERIAL/METHODS: The investigation was performed in 24 sexually mature femaleChinchilla rabbits divided into 4 groups of 6 rabbits each. The animals were oophorectomized (hypoprogesterone/hypoestrogen),hyperestrogen (sham surgery plus estradiol injections), hyperprogesterone (sham surgery plus 17 alpha-hydroxyprogesteroneinjections), or normal (sham surgery). Twelve weeks later, thiopental (40 mg/ml) was infused throughthe intravenous cannula at a constant rate (90 ml/hour) until loss of the eyelid reflex, at which timeblood was sampled for determination of thiopental, b-estradiol, progesterone and 17 alpha-hydroxyprogesteroneconcentrations. The ANOVA and Tukey tests were applied in statistical analysis (p=0.05). RESULTS: Hyperprogesteronerabbits lost consciousness faster (138 +/- 34.6 sec), at a lower plasma thiopental concentration (46.3+/- 6.6 microg/ml), and required less thiopental (24.63 +/- 6.44 mg/kg) than controls; hypoprogesteronerabbits lost consciousness slower (207.5 +/- 30.9 sec), at a higher plasma thiopental concentration (129.2+/- 24.9 microg/ml), and required more thiopental (38.51 +/- 2.33 mg/kg) than controls. The time of sleepinduction in the control group was 190 +/- 25.7 sec.; the serum thiopental concentration was 77.8 +/-13.9 microg/ml, and the total thiopental consumption was 35.8 +/- 3.51 mg/kg. Estrogen status has noeffect. CONCLUSIONS: Various hormonal states are accompanied by different CNS reactions to thiopental.Among the studied groups of sex steroids, only progestins significantly modify the CNS response to barbiturateinfusion.
Keywords: 17-alpha-Hydroxyprogesterone, Anesthetics, Intravenous, Central Nervous System, Eyelids, Gonadal Disorders, Hypogonadism, Progestins, Reflex, Thiopental, Time Factors
Editorial
01 March 2024 : Editorial
Editorial: First Regulatory Approvals for CRISPR-Cas9 Therapeutic Gene Editing for Sickle Cell Disease and Transfusion-Dependent β-ThalassemiaDOI: 10.12659/MSM.944204
Med Sci Monit 2024; 30:e944204
In Press
21 Feb 2024 : Clinical Research
Potential Value of HSP90α in Prognosis of Triple-Negative Breast CancerMed Sci Monit In Press; DOI: 10.12659/MSM.943049
22 Feb 2024 : Review article
Differentiation of Native Vertebral Osteomyelitis: A Comprehensive Review of Imaging Techniques and Future ...Med Sci Monit In Press; DOI: 10.12659/MSM.943168
23 Feb 2024 : Clinical Research
A Study of 60 Patients with Low Back Pain to Compare Outcomes Following Magnetotherapy, Ultrasound, Laser, ...Med Sci Monit In Press; DOI: 10.12659/MSM.943732
26 Feb 2024 : Clinical Research
Predictive Value of Combined HbA1c and Neutrophil-to-Lymphocyte Ratio for Diabetic Peripheral Neuropathy in...Med Sci Monit In Press; DOI: 10.12659/MSM.942509
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952