01 July 2005 : Extended abstract from EFAS 2011
Liquid-liquid systems for acid hydrolysis of glycoalkaloids from Solanum tuberosumL. tuber sprouts and solanidine extraction.
Nada C Nikolic, Mihajlo Z Stankovic, Dejan Z MarkovicMed Sci Monit 2005; 11(7): BR200-205 :: ID: 16971
Abstract
Background: Potato sprouts(Solanum tuberosum L.) contain steroidal glycoalkaloidscontaining solanidine, an important precursor for hormone synthesis. Glycoalkaloids are reported to inactivatethe Herpes simplex, Herpes zoster and Herpes genitalis viruses in humans, while Aglyones, including solasodine,may protect against skin cancer. Extracts of glycoalkaloids or solanidine can be used to obtain a potentialskin cancer preparation for clinical research. Material/Methods: Dried potato sprouts were used to obtainglycoalkaloids and solanidine. The hydrolysis of glycoalkaloids in a liquid-liquid system was performedusing a reflux condenser, obtaining extracts of glycolakaloids from dried and milled potato tuber sprouts.Hydrochloric acid was then added to the extract to form the first (aqueous) phase, and chloroform, trichloroethyleneor carbon tetrachloride to form the second (organic) phase of the liquid-liquid system. In this way,glycoalkaloid hydrolysis to solanidine and solanidine extraction in the organic liquid phase were combinedinto a single step. IR and GC/MS analysis of solanidine was also conducted. Results: Based on the resultswe obtained, the optimal liquid-liquid system was found to be 2% w/v hydrochloric acid in a 50% (volume)methanolic extract of glycoalkaloids from tuber sprouts, as the first phase, and chloroform as the secondphase. Using this system, a yield of 1.46 g solanidine per 100 g of dried potato sprouts can be achieved.Conclusions: Glycoalkaloid hydrolysis in a liquid-liquid system yields the aglycone solanidine can beobtained from dried potato sprouts. The yield of solanidine is higher than that obtained using solid-liquid-liquidsystems for glycoalkaloid hydrolysis from potato vines.
Keywords: Plant Shoots - chemistry, Solanaceous Alkaloids - chemistry, Solanaceous Alkaloids - isolation & purification, Chloroform, Diosgenin, Hydrochloric Acid, Hydrolysis, Methanol, Plant Extracts - isolation & purification, Plant Shoots - chemistry, Solanaceous Alkaloids - isolation & purification, Solanum tuberosum - chemistry, Solvents
Editorial
01 April 2024 : Editorial
Editorial: Forty Years of Waiting for Prevention and Cure of HIV Infection – Ongoing Challenges and Hopes for Vaccine Development and Overcoming Antiretroviral Drug ResistanceDOI: 10.12659/MSM.944600
Med Sci Monit 2024; 30:e944600
In Press
06 Mar 2024 : Clinical Research
Comparison of Outcomes between Single-Level and Double-Level Corpectomy in Thoracolumbar Reconstruction: A ...Med Sci Monit In Press; DOI: 10.12659/MSM.943797
21 Mar 2024 : Meta-Analysis
Economic Evaluation of COVID-19 Screening Tests and Surveillance Strategies in Low-Income, Middle-Income, a...Med Sci Monit In Press; DOI: 10.12659/MSM.943863
10 Apr 2024 : Clinical Research
Predicting Acute Cardiovascular Complications in COVID-19: Insights from a Specialized Cardiac Referral Dep...Med Sci Monit In Press; DOI: 10.12659/MSM.942612
06 Mar 2024 : Clinical Research
Enhanced Surgical Outcomes of Popliteal Cyst Excision: A Retrospective Study Comparing Arthroscopic Debride...Med Sci Monit In Press; DOI: 10.12659/MSM.941102
Most Viewed Current Articles
17 Jan 2024 : Review article
Vaccination Guidelines for Pregnant Women: Addressing COVID-19 and the Omicron VariantDOI :10.12659/MSM.942799
Med Sci Monit 2024; 30:e942799
14 Dec 2022 : Clinical Research
Prevalence and Variability of Allergen-Specific Immunoglobulin E in Patients with Elevated Tryptase LevelsDOI :10.12659/MSM.937990
Med Sci Monit 2022; 28:e937990
16 May 2023 : Clinical Research
Electrophysiological Testing for an Auditory Processing Disorder and Reading Performance in 54 School Stude...DOI :10.12659/MSM.940387
Med Sci Monit 2023; 29:e940387
01 Jan 2022 : Editorial
Editorial: Current Status of Oral Antiviral Drug Treatments for SARS-CoV-2 Infection in Non-Hospitalized Pa...DOI :10.12659/MSM.935952
Med Sci Monit 2022; 28:e935952